International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Implications of prophetic factors and their potential role in the development of radicular cyst

By: Arif Malik, Ayesha Zahid, Shahzad Ahmad, Sulayman Waquar, Muhammad Abdul Basit Ashraf

Key Words: LPS (Lipopolysaccharides), iNOS (Inducible nitric Oxide synthase), NO (Nitric Oxide), SOD (Superoxide dismutase), Radicular cyst (RC), Advanced oxidation protein products (AOPPs), Matrix metalloproteinases (MMPs)

Int. J. Biosci. 10(2), 201-209, February 2017.

DOI: http://dx.doi.org/10.12692/ijb/10.2.201-209

Abstract

Radicular cyst (RC) is the most common endodontic and inflammatory disease encompasses damage of periradicular tissues and resorption of bone adjacent to root of exaggerated teeth. Inflammatory cytokines uphold cyst growth and bone resorption as they are responsible to modulate bone remodeling. Reactive oxygen species (ROS) and nitric oxide derived from macrophages after bacterial lipopolysaccharides (LPS) may enhance bone loss by boosting the cytokine induced matrix metalloproteinases (MMPs) production in osteoblasts. Forty samples of radicular cysts and twenty from normal pulp were taken from extracted teeth (As control). Glutathione (GSH), Glutathione reductase (GRx), Glutathione peroxidase (GPx), Catalase (CAT), Malondialdehyde (MDA), superoxide dismutase (SOD), Vitamin-C, Vitamin-E, Nitric oxide (NO), Lipopolysaccharides (LPS), advanced oxidation protein products (AOPPs), advanced glycation end products (AGEs) were estimated spectrophotometrically. Inducible nitric oxide synthase (iNOS) and matrix metalloproteinases (MMPs) activity was measured in supernatants by kit method from tissue homogenates. Significantly (<0.05) decreased levels of antioxidants (SOD, CAT, GSH, vitamin C, vitamin E, GPx and GRx) were observed in tissue homogenate of radicular cyst as compared to healthy controls. Higher levels of MDA, AOPPs, AGEs, NO, iNOS LPS and MMP-9 signifies their importance and role in the development/expansion of radicular cyst. Inducible nitric oxide synthase (iNOS) play a key role to stimulate and release of elevated nitric oxide levels in an environment provided by tumor necrosis factor alpha. Lipopolysaccharide directly activates the host cells to secrete MMPs which play a key role in degrading the bone matrix and hence expansion of radicular cyst.

| Views 34 |

Implications of prophetic factors and their potential role in the development of radicular cyst

Benzie IF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239, 70-76.

Brannan PA, Thomas GJ, Langdon JD. 2003. The role of nitric oxide in oral diseases. Arch Oral Biol 48, 93-100.

Cekici A, Kantarci A, Hasturk H, Thomas E. 2014. Inflammatory and immune pathways in the pathogenesis of periodontal disease. Periodontol 64(1), 57-80.

Dezerega A, Osorio C, Madones J. 2010. Monocyte chemotactic protein-3: possible involvement in apical periodontitis chemotaxis. Int Endod J 43, 902-908.

Duque GA, Descoteaux A. 2014. Macrophage cytokines: involvement in immunity and infectious diseases. Front Immunol 5, 491.

Formigli L, Orlandini SZ, Tonelli P, Giannelli M, Martini M, Brandi ML, Bergamini M, Orlandini GE. 1995. Osteolytic processes in human radicular cysts: morphological and biochemical results. J Oral Pathol Med 24(5), 216-220.

Gelisgen R, Genc H, Kayali R, Oncul M, Benian A, Guralp O. 2011. Protein oxidation markers in women with and without gestational diabetes mellitus: a possible relation with paraoxonase activity. Diabetes Res Clin Pract 94, 404-409.

Graunaite I, Lodiene G, Maciulskiene V. 2011. Pathogenesis of apical periodontitis: a review. Oral Maxillofac J 2, 4-1.

Graves DT, Oates T, Gustavo PG. 2011. Review of osteo-immunology and the host response in endodontic and periodontal lesions. Journal of oral microbiology 3, 2000-2297.

Hama S, Takeichi O, Hayashi M, Komiyama K, Ito K. 2006. Co-production of vascular endothelial cadherin and inducible nitric oxide synthase by endothelial cells in periapical granuloma. Int Endod J 93, 179-184.

Hasturk H, Kantarci A, Thomas EVD. 2012. Oral Inflammatory Disease and Systemic inflammation: Role of the Macrophage. Front Immunol 3, 118.

Janda J, Work E. 1971. Colorimetric estimation of lipopolysaccharides. FEBS Letters 16(4), 343-345.

Khalifa GA, Shokier HM, Abo-Hager EA. 2010. Evaluation of neoplastic nature of keratocystic odontogenic tumor versus ameloblastoma. J Egypt Natl CancInst 22, 61-72.

Krafts KP. 2010. Tissue Repair: The hidden drama. Organogenesis 6, 225-233.

Lin LM, Huang GT, Rosenberg PA. 2007. Proliferation of epithelial cell rests, formation of apical cysts, and regression of apical cysts after peri-apical wound healing. J Endod 33(8), 908-916.

Loreto C, Galanti C, Leondardi R. 2013. Possible role of apoptosis in the pathogensis and clinical evolution of radicular cyst: an Immunohistochemical study. IntEndod J 46, 642-648.

Masanori H, Hayakawa Y, Kosugi H, Koizumi F. 1998. Localization of mRNA for inflammatory cytokines in radicular cyst tissue by in situ hybridization and induction of inflammatory cytokines by human gingival fibroblasts in response to radicular cyst contents. J Oral Pathol Med 27, 399-404.

Matsumoto MA, Ribeiro DA. 2007. Inducible nitric oxide expression correlates with the level of inflammation in periapical cysts. Eur J Dent 1, 212-215.

Mc Kee M, Scavone C, Nathanson JA. 1994. Nitric oxide, c GMP, and hormone regulation of active sodium transport. Proceedings of the National Academy of Sciences, USA 91, 12056-12060.

Meliou E, Kerezoudis N, Tosios K, Kiaris H. 2010. Notch 1 receptor, delta 1 ligand and HES 1 transcription factor are expressed in the lining epithelium of periapical cysts (pre-liminary study). Open Dent J 4, 153-158.

Nair PN. 2006.  On the causes of persistent apical periodontitis: a review. Int Endod J 39, 249-281.

Novo E, Parola M. 2008. Redox mechanisms in hepatic chronic wound healing and fibrogenesis. Fibrogenesis Tissue Repair 13(1), 5.

 

Schaller MI, Boeld U, Oberbauer S, Hamm G, Hube B, Korting HC. 2004. Polymorphonuclear leukocytes (PMNs) induce protective Th1-type cytokine epithelial responses in an in vitro model of oral candidosis. Microbiology 150(9), 2807-2813.

Takeichi O, Saito I, Okamoto Y, Tsurumachi T, Satio T, Saito T. 1998. Cytokine regulation on the synthesis of nitric oxide in vivo by chronically infected human polymorphonuclear leucocytes. Immunology 93, 275-80.

Waddington RJ, Moseley R, Embery G. 2000. Reactive oxygen species: a potential role in the pathogensis of periodontal disease. Oral diseases J 6, 138-151.

Wang T, Liu Q, Zhou L, Yuan JB, Lin X, Zeng R, Liang X, Zhao J, Xu J. 2015. Andrographolide Inhibits Ovariectomy-Induced Bone Loss via the Suppression of RANKL Signaling Pathways. Int J MolSci 16(11), 27440-27481.

Yalei WU, Humphrey MB, Nakamura MC. 2009. Osteoclasts-the innate immune cells of the bone. Autoimmunity 41(3), 183-194.

Arif Malik, Ayesha Zahid, Shahzad Ahmad, Sulayman Waquar, Muhammad Abdul Basit Ashraf.
Implications of prophetic factors and their potential role in the development of radicular cyst.
Int. J. Biosci. 10(2), 201-209, February 2017.
http://www.innspub.net/ijb/implications-prophetic-factors-potential-role-development-radicular-cyst/
Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update