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Heavy Metal and Antibiotic Resistant Bacteria Isolated from Soil Contaminated by the Sugar Industry Effluent

By: Saurabh Saini, Shailja Pant

Key Words: Antibiotic resistance, Heavy metal resistance, 16S rDNA, Multi-Drug Resistant, Sugar industry effluent.

Int. J. Biosci. 8(2), 36-46, February 2016.



In this study, antibiotic and heavy metal resistant bacteria were isolated from the agriculture field’s soil around sugar industry situated in Roorkee, District Haridwar, Uttarakhand (India).The antibiotic and heavy metal resistance profiles of the isolates were determined. Fifteen antibiotics and five heavy metals were used for reference. Four promising isolates were recovered, two gram positive E-2 and E-10 and two gram negative isolate E-7 and E-9. Detailed morphological, biochemical, and molecular characterization was done to identify the bacteria. The isolates E-2 and E-10 were identified as Bacillus licheniformis sp, and Teribacillus aidingensis sp. while E-7 and E-9 were identified as Chryseobacterium indologenes sp., and Enterobacter cloacae sp. respectively. These isolates were found resistant to heavy metals such as Ni2+, Pb2+, Cu2+, Zn2+ and Hg2+ at different concentrations ranging from 0.05 mM to 12 mM. The trend for tolerance of heavy metals by isolates was as follows: E-2:- Ni = Cu >Pb> Zn > Hg, E-7: Ni > Cu = Zn >Pb> Hg, E-9:- Pb = Ni > Cu = Zn > Hg and E-10:- Zn > Ni > Cu >Pb> Hg. The Anti-biogram pattern indicates that all isolates were showing resistance against more than five antibiotics and all are designated as multi-drugs resistant bacteria. These bacteria can be explored for bio-absorption of heavy metals from contaminated sites.


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