International network for natural sciences – research journal
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Gibberellin and indole acetic acid production capacity of endophytic fungi isolated from ZEA MAYS L.

By: Ismail, Muhammad Hamayun, Aqib Sayyed, Islam Ud Din, Humaira Gul, Anwar Hussain

Key Words: Endophytic fungi, IAA, Gibberellin, Zea mays.

Int. J. Biosci. 8(3), 35-43, March 2016.

DOI: http://dx.doi.org/10.12692/ijb/8.3.35-43

Abstract

Endophytic fungi are endosymbionts of majority of plants and having role in plant growth promotion by producing important secondary metabolites such as gibberellin and auxin. A total of 29 different endophytic fungal strains were isolated from the roots (21) and leaves (8) of Zea mays L. and screened for GA and IAA producing capacity. Out of 29 fungal strains, 18 were found to be growth promoters and 6 growth inhibitors of rice seedlings. MR 1-3-1 and MR 2-4-4 were the best shoot growth promoter strains. The presence of plant growth promoting hormone was later on confirmed using double beam spectrophotometer. For the GA3 detection and quantification, a very simple bioassay was done using wheat seeds with embryo removed. Maximum GA3 (52.26 ng/mL) was found in the culture filtrate of MR 2-4-4. Similarly maximum IAA (2.02 µg/mL) was detected in the culture filtrate of ML 1-5. The present work suggests that these endophytic fungi can be used as bio-fertilizer in the agricultural fields because extensive use of artificial fertilizers, cause deterioration of environment and have hazardous effects for plants. Moreover, artificial fertilizers are very costly and must be supplied continuously to the fields while endophytic fungi stay in the field for years and safe to the plant and environment.

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