Genotyping of Helicobacter pylori isolates from Egyptian Patients
By: Eman M. Abd El Azeem, Abdel-Rahman B. Abdel-Ghaffar, Maha Hassan Shokaeir, Hend Abdelmoniem Ali
Key Words: Helicobactor pylori, cagA genotyping, babA2 , Gastritis, Peptic ulcer
Int. J. Biosci. 10(4), 121-128, April 2017.
The cag AandbabA2genes are the common virulence determinants of Helicobactor pylori (H. pylori). Acquiring virulent strains of H. pylori is associated with increased risk for the development of gastric ulcers or cancer. This study was carried out to investigate the prevalence of cytotoxin associated gene A (cagA) and blood adhesion binding antigen (babA2) genotypes of H. pylori isolates from Egyptian patients and its correlation with the sensitivity to antibiotics included in triple therapy. Culture of stool specimens was performed in stool samples obtained from 60 patients positive for H. pylori antigen in stool. DNA was extracted from H. pylori pure cultures. Genotyping was performed by PCR, using specific primers for, cagA and babA2 genes. Disc diffusion method was used to determine the sensitivity of cultured bacteria to amoxillin (AMX), metronidazole (MTZ) and clarithromycin (CLM). cagA was detected in 34.78% of isolates, while the bab7 allele of babA2 gene was detected in 43.47%isolates.13% of strains were genotype A (cagA+/babA2+), 21.7% of strains were genotype B (cagA+/babA2-), 30.4 % of strains were genotype C (cagA-/babA2+) and 34.8%of strains were genotype D (cagA-/babA2-). The highest prevalence of sensitivity was for AMX 52.2%, while MTZ was the lowest sensitivity prevalence 30.4%, when compared to the mean sensitivity prevalence of both concentrations of CLR (39.15%). According to these finding Genotyping combination (cagA-/babA2-) is the most prevalent and MTZ is not the optimal choice for the treatment of Egyptian patients and should not be included in the treatment regimens for H. pylori in Egypt.
Genotyping of Helicobacter pylori isolates from Egyptian Patients
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