Genetic transformation of an elite commercial wheat variety through Agrobacterium tumefaciens
By: Muhammad Ali, Muhammad Tahir, Shahid Hameed
Key Words: Wheat, plant transformation, immature embryos, BYDV.
Int. J. Biosci. 8(1), 17-24, January 2016.
Wheat (Triticum aestivum L.) is the leading food grain crop of Pakistan that contributes 2.7 percent to the GDP. However, the crop is significantly affected by Barley yellow dwarf virus (BYDV). To combat with, introduction of virus-derived gene(s), through genetic engineering, is a powerful tool to confer resistance. Wheat is considered recalcitrant to be transformed genetically. The success regarding genetic transformation is almost limited to the cv. Bobwhite. Bobwhite, however, is not an isogenic line and may be used as a transgenic crossing material. Crossing for a transgenic trait from Bobwhite may be complicated by a linkage drag. Alternatively, targeting elite variety for transformation will be more economical. Considering transformation of an elite variety “Sehar-2006” via Agrobacterium tumefaciens (LBA4404), optimization of protocol harboring binary plasmid construct (pING71/BYDV-CP19) with nptII marker gene has been discussed.