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Generation and characterization of chicken egg yolk rotavirus antivac IgY antibodies for its prospective use in oral passive therapy

By: Brindha Chelliappan, M. Tamilmozhi, Sentila Rajan, Michael Antonysamy

Key Words: Rotavirus, Gastroenteritis, IgY antibodies, Passive therapy, Diarrhoea

Int. J. Biosci. 11(5), 235-244, November 2017.

DOI: http://dx.doi.org/10.12692/ijb/11.5.235-244

Certification: ijb 2017 0150 [Generate Certificate]

Abstract

Among diarrheal diseases in developing countries, Rotavirus gastroenteritis plays the major reason for infant mortality and hospitalizations in India under 5years of age. In India, nearly 80,000 to 1lakh children die and 9lakh hospitalizations, 32.7 lakh hospital visits happen yearly due to this infection. Since the incidence rate is very high and only oral rehydration therapy is available, there is an urgent need for an alternative therapy to treat the infection. Antibody therapy proves to be an effective alternate oral therapy to treat the rotavirus infection. Chicken Egg yolk antibodies against the vaccine strain of rotavirus were generated by immunizing white leghorn chicken intramuscularly. Antivac IgY antibodies were purified by PEG precipitation method followed by Dialysis. Total protein content of the purified antibodies was found to be 40.96 mg/ml and the total IgY antibodies were found to be 14.91 mg/ml. Also, the purified Antivac IgY antibodies correspond to 180kDa protein as visualized in SDS PAGE Analysis. The antibody titer was around 1:50,000 as determined by Indirect ELISA method. The antibodies were stable up to the temperature of 40°C and the activity started diminishing above 60°C.The antibody activity was affected by acidic pH of 3 and below and the activity was diminished to an extent in the alkaline conditions of pH 9 and above. The activity was unaffected between pH 4 to pH 8.0. Hence, these Antivac IgY antibodies can be used in passive oral immunotherapy for treating a rotaviral infection.

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Generation and characterization of chicken egg yolk rotavirus antivac IgY antibodies for its prospective use in oral passive therapy

Adham M, Abdou, Manal ME, Ahmed, Yusuke Yamashita, Mujo Kim. 2014. Immunoglobulin: A Natural Way to Suppress Helicobacter pyloriin Humans. Health, 6, 781-791.

http://dx.doi.org/10.4236/health.2014.68100

Celina G. Vega, Marina Bok, Anastasia N. Vlasov0a, Kuldeep S. Chattha, Fernando M. Fernandez, Andre´ S. Wigdorovitz, Viviana G. Parren˜o, Linda J. Saif. 2012. IgY Antibodies Protect against Human Rotavirus Induced Diarrhea in the Neonatal Gnotobiotic Piglet Disease Model. PLoS ONE 7(8), e42788.

https://doi.org/10.1371/journal.pone.0042788

Chao-Yang Fu, He Huang, Xiao-Mei Wang A, Yong-Gang Liu, Zhi-Guo Wang, Shang-Jin Cui, Hong-Lei Gao, Zan Li, Jing-Peng Li, Xian-Gang Kong. 2006. Preparation and evaluation of anti-SARS coronavirus IgY from yolks of immunized SPF chickens. Journal of virological methods, 133(1), 112-115.

http://dx.doi.org/10.1016/j.jviromet.2005.10.027

David Carlander, Hans Kollberg, Per-Erik Wejaker, Anders Larsson. 2000. Peroral immunotherapy with yolk antibodies for the prevention and treatment of enteric infections.

Immunologic research 21(1), pg 1-6.

https://dx.doi.org/10.1385/IR:21:1:1

Diana Pauly, Pablo A. Chacana, Esteban G. Calzado, Björn Brembs, Rüdiger Schade. 2011. IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation. Journal of Visualized Experiments (51), e3084.

www.jove.com/video/3084/igy-technology-extraction-chicken-antibodies-from-egg-yolk

Harlow E, Lane D. 1988. Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

https://books.google.co.in/books?id=0p29pFaLwR8C&lpg=PP1&dq=Harlow%2C%20E.%20and%20Lane%2C%20D.%201988.%20Antibodies%3A%20A%20Laboratory%20Manual.&pg=PP1#v=onepage&q&f=false

Ji-Hyun Shin, Mierha Yang, Seung Woo Nam, Jung Taik Kim, Na Hye Myung, Won-Gi Bang, Im Hwan Roe. 2002. Use of egg yolk-derived immunoglobulin as an alternative to antibiotic treatment for control of Helicobacter pylori infection. Clinical and Diagnostic Laboratory Immunology 9(5), 1061-1066.

http://cvi.asm.org/content/9/5/1061?relatedurls=yes&legid=cdli;9/5/1061

http://dx.doi.org/10.1128/CDLI.9.5.1061-1066.2002

Kazuyuki Hirai, Hideyuki Arimitsu, Koji Umeda, Kenji Yokota, Lianhua Shen, Kiyoshi Ayada, Yoshikatsu Kodama, Takao Tsuji, Yoshikazu Hirai, Keiji Oguma. 2010. Passive oral immunization by egg yolk immunoglobulin (IgY) to Vibrio cholerae effectively prevents cholera. Acta Med Okayama. 2010 Jun 64(3), 163-70.

https://dx.doi:10.18926/AMO/40008

King J, Laemmli UK. 1971. Polypeptides of the tail fibres of bacteriophage T4. Journal of molecular biology, 62(3), 465IN1473-472IN6477.

https://doi.org/10.1016/0022-2836(71)90148-3

Lee KAChang SKLee YJLee JHKoo NS. 2002. Acid stability of anti-Helicobacter pylori IgY in aqueous polyol solution. Journal of Biochemistry and Molecular Biology.Sep 30, 35(5), 488-93.

www.koreascience.or.kr/search/articlepdf_ocean.jsp?admNo=E1MBB7_2002_v35n5_488

Marion M Bradford. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2), 248-254.

www.sciencedirect.com/science/article/pii/0003269776905273

Polson A, Coetzer T, Kruger J, Von Maltzahn, E, Van der Merwe KJ. 1985. Improvements in the isolation of IgY from the yolks of eggs laid by immunized hens. Immunological investigations,  14(4), 323-327.

http://dx.doi.org/10.3109/08820138509022667

Reschová S, Franz J, Štěpánek J, Rozkošná A. 2000. Immunochromatographic detection of bovine rotavirus using egg yolk antibodies.  Veterinární Medicína 2000 45(2), 33-37 ref.28.

Thirumalai Diraviyam, Bin Zhao, Yuan Wang, Ruediger Schade, Antonysamy Michael, Xiaoying Zhang. 2014. Effect of Chicken Egg Yolk Antibodies (IgY) against Diarrhea in Domesticated Animals: A Systematic Review and Meta-Analysis. PLoS ONE 9(5), e97716.

www.ncbi.nlm.nih.gov/pmc/articles/PMC4028221/pdf/pone.0097716.pdf

http://dx.doi.org/10.1371/journal.pone.0097716

Yuan-e Yanga, Junlin Wena, Suqing Zhaoa, Kun Zhang, Yingliang. 2014. Prophylaxis and therapy of pandemic H1N1 virus infection using egg yolk antibody. Journal of virological methods, 206, 19-26.

http://dx.doi.org/10.1016/j.jviromet.2014.05.016

Ziad Jaradat, Ronald R Marquardt. 2000. Studies on the stability of chicken IgY in different sugars, complex carbohydrates, and food materials. Food and agricultural immunology 12(4), 263-272.

http://dx.doi.org/10.1080/09540100020008137

Brindha Chelliappan, M. Tamilmozhi, Sentila Rajan, Michael Antonysamy.
Generation and characterization of chicken egg yolk rotavirus antivac IgY antibodies for its prospective use in oral passive therapy.
Int. J. Biosci. 11(5), 235-244, November 2017.
http://www.innspub.net/ijb/generation-characterization-chicken-egg-yolk-rotavirus-antivac-igy-antibodies-prospective-use-oral-passive-therapy/
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