Extracellular enzymatic activity, phytochemical analysisand in vitro biological assessment of endophytic fungi isolated fromTaxus fauna
By: Hira Mehboob Mirza, Muniba Jadoon, Nighat Fatima, Fariha Hasan, Safia Ahmed, Masoom Yasinzai
Key Words: Taxus fauna, Plectaniamilleri, Trichoderma asperellumT77, Endophytic fungi, Extracellular enzymes.
Int. J. Biosci. 8(2), 82-94, February 2016.
The present study was aimed to investigate biological activities and phytochemical profiling of endophytic fungi isolated from Taxus fauna. A total of two endophytic strainsPlectaniamilleri(NFL1) andTrichoderma asperellum T77 (NFL2) were selected and 8 samples were prepared by solid state fermentation on four different media (PDA, SDA, TM, Rice). Phytochemical profiling was conducted by analyzing total phenolic and total flavonoid contents colorimetricallyand the samples were biologically evaluated by employing antimicrobial, antileishmanialand several multimode antioxidant assays. The maximum phenolicand flavonoid contents was observed in NFL1-PDA (3.12 ± 0.10 µg GAE/mgE)(1.40±0.05 µg QE/mgE) and NFL2-PDA (3.403 ± 0.10 µg GAE/mgE) (2.6 ± 0.10 µg QE/mgE) extracts respectively. Maximum antioxidant and reducing power potential was displayed by NFL1-PDA, NFL2-PDA (25.4±0.58, 27.94±0.50 µg AAE/mg E) and NFL1-TM, NFL2-SDA (15.4 ± 0.35, 17.41 ± 0.30 µg AAE/mg E) respectively.Highest DPPH radical scavenging activity was displayed by NFL1-PDA (79.2±2.4) and NFL2-PDA (77.5±18) extracts. Significant antibacterial activity was also observed by NFL1-PDA and NFL2-PDA against all the tested bacterial strains. Remarkable antileishmanial activity was also manifested by NFL1-PDA extract (IC50 1.5 µg/mL).P milleri (NFL1) was found positive for the production of amylase, pectinase laccase and lipase while T. asperellum(NFL2) tested positive for laccase and amylase.The results of presentstudy show that endophytic fungi associated with Taxus faunaare potential source of antimicrobial and antioxidant agents and could be exploited for the production of industrially important enzymes.