International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Exploring bacterial flora in rhizosphere of Nicotiana tabbacum at district Swabi; High tobacco production zone, Pakistan

By: Muhammad Idrees, Kalsoom, Shahzeb Wahid, Sana Mateen, Amar, Faheem Ahmad Khan, Johar Jamil

Key Words: Nicotiana tabacum, Rhizosphere, Bacterial population

Int. J. Biosci. 11(5), 186-191, November 2017.

DOI: http://dx.doi.org/10.12692/ijb/11.5.186-191

Certification: ijb 2017 0144 [Generate Certificate]

Abstract

Nicotiana tabbacum is an annually grown herbaceous plant promising great economic importance and the rhizosphere of Nicotiana tabbacum harbours considerable groups of microorganisms. The aim of research was to isolate and study the growth characteristics of indigenous bacterial population from the rhizosphere of Nicotiana tabbacum from different areas of district Swabi i.e. Marghuz, Kotha and Zaida and to maintain pure cultures for future reference. The soil samples were collected from rhizosphere with different age groups of the same species and brought into the lab for further processing. All samples were grown on different Media including Nutrient agar, MacConkey agar and Mannitol Salt Agar and a series of biochemical tests were performed for identification and characterization of the most commonly occurring rhizobacteria of this region. The result of colony morphology and biochemical tests confirmed the presence of four bacterial isolates i.e. Micrococcus luteus, Bacillus subtilis, Bacillus cereus and Escherichia coli. Bacillus subtilis, Bacillus cereus and Micrococcus luteus are gram positive while Escherichia coli is a gram negative bacteria. It is inferred from this study that most common isolates in rhizosphere of Nicotiana tabbacum was antibiotic producers which can be used as biofertilizer for enhancing growth and prevention of pathogens

| Views 109 |

Exploring bacterial flora in rhizosphere of Nicotiana tabbacum at district Swabi; High tobacco production zone, Pakistan

Hiltner L. 1904. Uber  neuere Erfahrungen and Probleme ouf dem Gebiet der Boden bakteriologie und unter besonderer Beruck sichtigung der Grundun geng and Brache. Arb. DtschLundvt- Ges 98(5), 9R.

Junaid A. 2011. Tobacco production: beating the average global yields.

www.tribune.com.pk/story/302317/tobaccoproduction-beating-the-average-global-yield/

Kaprelyants AS, Kell DB. 1993. Dormancy in stationary-phase cultures of Micrococcus luteus: Flow cytometric analysis of starvation and resuscitation. Applied and Environmental Microbiology 59(10), 10-3187.

Orhan E, Esitken A, Ercisli S, Turan M, Sahin F. 2006. Effects of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient contents in organically growing raspberry. Scientia Horticulturae, 111 (suppl 1), 38-43.

Narula N, Kothe E, Behl RK. 2009. Role of root exudates in plant-microbe interactions. Journal of Applied Botany. Food Quality, 82(2), 122-130.

Naseer QA, Kalsoom Jamil J, Khan H, Jalil F. 2017. Screening an antifungal efficacy of indigenous bacteria from rhizosphere of Periploca apylla. International journal of Biosciences, 11(1), 351-359

Raaijmakers JM, Weller DM. 2001. Exploiting genotypic diversity of 2,4-diacetyl phloro glucinol producing Pseudomonas spp.: characterization of superior root-colonizing P. fluorescens strain Q8r1-96. Applied Environmental Microbiology. 67, 2545-2554.

Ryan KJ, Ray CG. 2004. Sherris Medical Microbiology.

Shamima N, Rahman MA. 2007. International Journal of Biological sciences 15, 77-82.

Sharman N, James GC. 2008. Microbiology Laboratory Manual. 7th Edition, Pearson education. 143-279.

Starkey RL. 1938. Some influences of the development of higher plants upon the microorganisms in the soil VI. Microscopic examination of the rhizosphere. Soil Science 45, 207-49.

Subhashini DV, Padmaja K. 2009a. Antifungal activity of Pseudomonas fluorescens against different plant pathogenic fungi of tobacco, Indian Journal of Plant Protection 37(1&2), 211-212.

Subhashini DV, Padmaja K. 2009b. Isolation of Streptomyces from tobacco soils that show antimicrobial activity. Journal of Biology Cont., 23(4), 417-419.

Warcup JH. 1950. Basic plant pathology methods. Nature London 166, 117.

Muhammad Idrees, Kalsoom, Shahzeb Wahid, Sana Mateen, Amar, Faheem Ahmad Khan, Johar Jamil.
Exploring bacterial flora in rhizosphere of Nicotiana tabbacum at district Swabi; High tobacco production zone, Pakistan.
Int. J. Biosci. 11(5), 186-191, November 2017.
http://www.innspub.net/ijb/exploring-bacterial-flora-rhizosphere-nicotiana-tabbacum-district-swabi-high-tobacco-production-zone-pakistan/
Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update