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Exploration of novel somatic Hspb1 mutations in widespread neoplasms of Canis familiaris

By: Rashid Saif, Ali Raza Awan, Leslie A. Lyons, Barbara Gandolfi, Muhammad Wasim

Key Words: Hspb1 mutations, Dog cancer, Tumor variations

Int. J. Biosci. 10(5), 265-272, May 2017.

DOI: http://dx.doi.org/10.12692/ijb/10.5.265-272

Abstract

Genotype-phenotype relationship is the matter of great concern in modern genetics, and exploring potential molecular markers through case control studies is considered to be a powerful diagnostic tool in field of comparative oncology. Eighteen cancers samples of Canis familiaris were genotyped through direct sequencing to ascertain the genetic alteration within Hspb1 gene. Total 17 polymorphic sites were observed in this gene, and most of the cancers are observed heterozygous on these altered loci of exon 1 and exon 3 region. Hspb1 gene, CDS locus 1349, 1407 and 1411 are homozygous, while CDS positions 148 and 1050 are mixed loci, and both are missense mutations. It is also observed that most of the cancer samples are heterozygous in intronic regions. These cancer-risk SNP markers may have significant role in diagnostic, prognostic and may be helpful hotspots in case-control association studies.

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Exploration of novel somatic Hspb1 mutations in widespread neoplasms of Canis familiaris

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Rashid Saif, Ali Raza Awan, Leslie A. Lyons, Barbara Gandolfi, Muhammad Wasim.
Exploration of novel somatic Hspb1 mutations in widespread neoplasms of Canis familiaris.
Int. J. Biosci. 10(5), 265-272, May 2017.
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