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Exploration of gastric ulcer in the people of rural area of Peshawar/KPK through enzyme linked immunosorbent assay

By: Bashir Ahmad, Komal Aman, Sajid Ali, Shumaila Bashir, Nourin Mahmood, Muhammad idrees

Key Words: H. Pylori, Gastric ulcer, ELISA, Gender, Age

Int. J. Biosci. 11(4), 365-371, October 2017.

DOI: http://dx.doi.org/10.12692/ijb/11.4.365-371

Certification: ijb 2017 0139 [Generate Certificate]

Abstract

Helicobacter pylori reside and grow in digestive tract of human and cause gastric ulcer which in extreme cases leads to gastric adinocarcinoma. People in most of the territories in Peshawar/Pakistan believe that H. pylori is due to different life style habits and different acidic food usage. Therefore to clear this contradiction and expose actual cause of this disease and to bring awareness among people, this study has been designed. H. pylori antibodies were determined using ELISA (Enzyme Linked Immunosorbent Assay), because this is an easy diagnostic approach and is low cost diagnostic test. This study was started from January 2016 to May 2016 and Blood sample was collected from general population of a rural area of Peshawar KPK. In this aspect we collected blood samples from 80 subjects who had stomach problem and also from 6 normal individuals as control. Gender and age base study was done in this research. Out of 80 patients, 60 patients showed positive results for H. pylori, 10 were negative and 10 showed equivocal (nor clearly positive neither negative). Moreover, female have shown more positivity as compared to male and 40-50 age group subjects were more affected. H. pylori infection is present in more than 50% of the population and bacteria are involved in causing it not people’s life style. Secondly huge population involvement reflects unawareness of the problem concerned.

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Exploration of gastric ulcer in the people of rural area of Peshawar/KPK through enzyme linked immunosorbent assay

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Bashir Ahmad, Komal Aman, Sajid Ali, Shumaila Bashir, Nourin Mahmood, Muhammad idrees.
Exploration of gastric ulcer in the people of rural area of Peshawar/KPK through enzyme linked immunosorbent assay.
Int. J. Biosci. 11(4), 365-371, October 2017.
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