International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Evaluation of thrombolytic, antimicrobial and cytotoxicity potential of extract and its polar fractions of Heliotropium strigosumby in-vitro assay

By: Abdur Rehman, Sami Ullah, Raja Adil Sarfraz, Faisal Nawaz, Abdul Qayyum, Muhammad Javid, Syed Muhammad Imran Bin Riaz, Khalil Ullah Khan

Key Words: Thrombolytic, Antimicrobial activities, Cytotoxicity, Polar fractions, Heliotropium strigosumby

Int. J. Biosci. 10(1), 109-116, January 2017.

DOI: http://dx.doi.org/10.12692/ijb/10.1.109-116

Abstract

Medicinal plants are the major sources of natural product and are known as natural source of derived compounds which are being used in the safety of human life. In the early age of human life, the plants are used for the nutritional purposes but as the research is focus on the investigation of their medicinal potential then these are become a useful source of remedies for various diseases and health improvements. The present work was focus on evaluation of antimicrobial, cytotoxicity and thrombolytic potential of methanolic extract and its polarities based fractions of Heliotropium strigosum. The extraction was performed by methanolic solvent and fractionation by using chloroform, ethyl acetate and aqueous solvent sequentially on the basis of their polarities. Antibacterial activity and antifungal activity was performed by disk inhibition assay which showed significant (p<0.05) potential 36mm and 32mm was shown by against Staphylococcus aurous and Escherichia coli respectively. Highest antifungal activity was shown by 28mm by aqueous fractions whereas least 12mm by ethyl acetate fraction against the Aspergilus nigerfungus species. Cytotoxicity activity also showed significant results by hemolytic assay. Thrombolytic activity showed highest activity (34.54%) by methanolic crude extract while ethyl acetate fraction showed lowest (23.16%) activity. This study showed that the crude extract and polar fractions of crude methanolic extract of H. strigosum can be considered the potential sources of antimicrobial and thrombolytic agent not only in the food industry but also in cosmetics and pharmaceutical preparations.

| Views 29 |

Evaluation of thrombolytic, antimicrobial and cytotoxicity potential of extract and its polar fractions of Heliotropium strigosumby in-vitro assay

Afzal M, Shahid M, Jamil A. 2014. Phytochemical spectrum of essential oil of Paganum harmala by GC-MS and antimicrobial activity using sequential solvents fractions and essential oil. Asian Journal of Chemistry 26, 574.

http://dx.doi.org/10.14233/ajchem.2014.159988

Alkjaersig N, Fletcher AP, Sherry S. 1959. The mechanism of clot dissolution by plasmin. Journal of Clinical Investigation 38, 1086.

http://dx.doi.org/10.1172/JCI1038855

Arslan R, Bor Z, Bektas N, Meriçli AH, Ozturk Y. 2011. Antithrombotic effects of ethanol extract of Crataegus orientalis in the carrageenan-induced mice tail thrombosis model. Thrombosis research, 127, 210-213.

http://dx.doi.org/10.1016/j.thromres.2010.11.028

Atawodi SE. 2005. Antioxidant potential of African medicinal plants. African Journal of Biotechnology 4, 128-133.

Barbour EK, Al Sharif M, Sagherian V. K., Habre AN, Talhouk RS, Talhouk SN. 2004. Screening of selected indigenous plants of Lebanon for antimicrobial activity. Journal of Ethnopharmacology 93, 1-7.

http://dx.doi.org/10.1016/j.jep.2004.02.0277

Collen D. 1990. Coronary thrombolysis: streptokinase or recombinant tissue-type plasminogen activator?. Annals of internal medicine, 112, 529-538.

http://dx.doi.org/10.7326/0003-4819-112-7-529

Devi JS, Bhimba BV. 2014. Antibacterial and Antifungal Activity of Silver nanoparticles Synthesized using Hypnea muciformis. Biosciences Biotechnology Research Asia, 11, 235-238.

Gesler WM. 1992.  Therapeutic landscapes: medical issues in light of the new cultural geography. Social science & medicine 34, 735-746.

http://dx.doi.org/10.1016/0277-9536(92)90360-3

Gülçın İ, Oktay M, Kıreçcı E, Küfrevıolu ÖI. 2003. Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extracts. Food chemistry 83, 371-382.

http://dx.doi.org/10.1016/S0308-8146(03)00098-0

Hamill FA, Apio S, Mubiru NK, Bukenya-Ziraba R, Mosango M, Maganyi OW, Soejarto DD. 2003. Traditional herbal drugs of Southern Uganda, II: literature analysis and antimicrobial assays. Journal of Ethnopharmacology, 84, 57-78. http://dx.doi.org/10.1016/S0378-8741(02)00289-1

Hussain S, Jamil M, Ullah F, Khan A, Ullah F, Arfan M, Khatoon L. 2010. Antimicrobial and antioxidant activities of the plant Heliotropium strigosum. African Journal of Biotechnology 9, 7738-7743.

Ivan S, Jozef F. 2011. Content of heavy metals in poppy seeds (Papaver somniferum L.). Advances in Environmental Biology 11, 496-501.

Javid M, Sarfraz RA, Nawaz F, Qayyum A, Rehman A, Aziz S, Riaz SMIB. 2016. Effect of Solvents on the biological activities and phenolic composition of Achyranthes aspera. International Journal of Biosciences 9, 145-153.

http://dx.doi.org/10.12692/ijb/9.6.145-153

Kumar BA, Lakshman K, Nandeesh R, Kumar, PA, Manoj B, Kumar V, Shekar DS. 2011. In vitro alpha-amylase inhibition and in vivo antioxidant potential of Amaranthus spinosus in alloxan-induced oxidative stress in diabetic rats. Saudi journal of biological sciences 18, 1-5.

http://dx.doi.org/10.1016/j.sjbs.2010.08.002

Qayyum A, Sarfraz RA, Ashraf A, Adil S. 2016. Phenolic composition and biological (anti diabetic and antioxidant) activities of different solvent extracts of an endemic  plant (Heliotropium strigosum). Journal of the Chilean Chemical Society 61, 2828-2831.

http://dx.doi.org/10.4067/S071797072016000100016

Rai PK, Jaiswal D, Singh RK, Gupta RK, Watal G. 2008. Glycemic properties of Trichosanthes dioica leaves. Pharmaceutical biology 46, 894-899.

http://dx.doi.org/10.1080/13880200802370167

Rasool N, Tehseen H, Riaz M, Komal R, Muhammad Z, Mahmood Y. 2013. Cytotoxicity studies and antioxidant potential of Acacia nilotica roots. Int J Chem Biochem Sci. 3, 34-41.

Riaz M, Rasool N, Bukhari IH, Shahid M, Zubair M, Rizwan K, Rashid U. 2012. In vitro antimicrobial, antioxidant, cytotoxicity and GC-MS analysis of Mazus goodenifolius. Molecules 17, 14275-14287.

http://dx.doi.org/10.3390/molecules171214275

Shao H, Huang X, Zhang Y, Zhang C. 2013. Main alkaloids of Peganum harmala L. and their different effects on dicot and monocot crops. Molecules 18, 2623-2634.

http://dx.doi.org/0.3390/molecules180326233

Xue-Ting LIU, Yao SHI, Liang JY, Zhi-Da MI. N. 2009. Antibacterial ent-rosane and ent-kaurane diterpenoids from Sagittaria trifolia var. sinensis. Chinese Journal of Natural Medicines 7, 341-345.

http://dx.doi.org/10.3724/SP.J.1009.2009.00345

Zuber M, Tabasum S, Jamil T, Shahid M, Hussain R, Feras KS, Bhatti KP. 2014. Biocompatibility and microscopic evaluation of polyurethane-poly (methyl methacrylate)- titnanium dioxide based composites for dental applications. Journal of Applied Polymer Science 131, 1-9.

http://dx.doi.org/10.1002/app.39806

Abdur Rehman, Sami Ullah, Raja Adil Sarfraz, Faisal Nawaz, Abdul Qayyum, Muhammad Javid, Syed Muhammad Imran Bin Riaz, Khalil Ullah Khan. 2017. Evaluation of thrombolytic, antimicrobial and cytotoxicity potential of extract and its polar fractions of Heliotropium strigosumby in-vitro assay. Int. J. Biosci. 10(1), 109-116.
http://dx.doi.org/10.12692/ijb/10.1.109-116
Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update