International network for natural sciences – research journal
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Evaluation of antibacterial activity of three flower colours Chrysanthemum morifolium Ramat. against multi-drug resistant human pathogenic bacteria

By: Dilruba Yeasmin, Rawnak Jahan Swarna, Mst. Samima Nasrin, Sarwar Parvez, Mohammad Firoz Alam

Key Words: Chrysanthemum morifolium, Antibacterial activity, Pathogenic bacteria, MIC, MBC.

Int. J. Biosci. 9(2), 78-87, August 2016.



The present investigation was carried out to evaluate the anitibacterial activity of flower extracts of three colours (pink, yellow and white) of Chrysanthemum morifolium Ramat. against five Gram positive bacteria viz., Staphylococcus aureus, Bacillus cereus, Streptococcus-β-haemolytica, Bacillus subtilis, Sarcina lutea and five Gram negative bacteria viz., Klebsiella sp, Klebsiella pneumonia, Pseudomonas aeruginosa, Salmonella typhi, Shigella dysenteriae. Between the two extracts (ethanol and methanol) the ethanol extracts of white flower was more effective than pink and yellow flower of C. morifolium. The ethanol extracts of white flower was showed the highest zone of inhibition (24.40 mm) against Shigella dysenteriae, the lowest MIC value (150 mg/ml) was against Shigella dysenteriae and Streptococcus-β-haemolytica and the lowest MBC value (200 mg/ml) was against Shigella dysenteriae. MIC and MBC of the extracts have ranged from 150-250 mg/ml and 200-300 mg/ml respectively. The lowest MIC and MBC values have observed against Shigella dysenteriae. For pink and yellow flower extracts, statistical results indicated that there are significant differences among bacterial species, solvent and bacterial strain, but no significant differences are shown in replication. But in case of yellow flower, there are significant differences among bacterial species, solvent, replication and bacterial strain. In addition, interaction between bacterial species and solvent appears to be significantly different.

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