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Evaluation of the antioxidant and anticancer activities of Canarium ovatum (Burseraceae) pulp extracts

By: Lilibeth A. Cajuday, Daile Meek S. Membreve, Jocelyn E. Serrano

Key Words: Antiangiogenic, Anticancer, Antioxidant, Canarium Ovatum, Cytotoxicity, Phytochemical screening.

Int. J. Biosci. 11(3), 247-256, September 2017.

DOI: http://dx.doi.org/10.12692/ijb/11.3.247-256

Certification: ijb 2017 0102 [Generate Certificate]

Abstract

The study evaluated the antioxidant and anticancer activities of Canarium ovatum pulp. Mechanical and ethanolic extractions were done to prepare the oil (COPO) and ethanolic (COPE) extracts from the C. ovatum pulp. The extracts were used to screen for the biological activities and phytochemical components. The antioxidant level was evaluated using the DPPH (2, 2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing ability of plasma) assays. The CAM (chorioallantoic membrane) and sea urchin fertilization assays were used to test for anticancer activity and cytotoxicity. Results of the DPPH and FRAP assays showed high reducing power and free radical scavenging activities of COPE compared to COPO and ascorbic acid. CAM assay revealed a significant inhibition (p=0.05) in blood vessel formation in all the COPE-treated samples only compared to the vehicle control. In the sea urchin fertilization assay, the inhibitory effect of COPE was observed in the medium (1mg/ml) and high (10mg/ml) treatments where there were significant reductions (p=0.05) in the percentage of fertilized egg compared with the control. The lowest concentration (0.1mg/ml) of COPE was inactive in this assay. The computed IC50 for COPE was 0.369mg/mL. Phytochemical screening of the different extracts revealed the presence of bioactive constituents such as alkaloids, flavonoids, glycosides, saponins, sterols, tannins and triterpenes. Further studies be done to validate the results and elucidate mechanism of action of the bioactive components present in the extracts.

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Evaluation of the antioxidant and anticancer activities of Canarium ovatum (Burseraceae) pulp extracts

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Lilibeth A. Cajuday, Daile Meek S. Membreve, Jocelyn E. Serrano.
Evaluation of the antioxidant and anticancer activities of Canarium ovatum (Burseraceae) pulp extracts.
Int. J. Biosci. 11(3), 247-256, September 2017.
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