Effect of Solvents on the biological activities and phenolic composition of Achyranthes aspera
By: Muhammad Javid, Raja Adil Sarfraz, Faisal Nawaz, Abdul Qayyum, Abdur Rehman, Shahid Aziz, Syed Muhammad Imran Bin Riaz
Key Words: Extraction, Antioxidant, Antibacterial, Phenolic compounds
Int. J. Biosci. 9(6), 145-153, December 2016.
Plants kingdom have a variety of species which are being used for the treatment of various disease in different parts of world including Asia. Synthetic compounds used in the treatment of have side effects and more costly. So it is need to find such compounds from natural sources which have less side effect and economical. So the present research work was focus on the evaluation of Achyranthes aspera regarding its antioxidant and antimicrobial potential by extracting in various solvents such as methanol, ethanol and aqueous. Aqueous solvent showed highest extraction yield 10.3% than other solvent. Antibacterial activity was performed by well diffusion assay. Aqueous extract showed maximum antibacterial (29mm and 23mm) activity against both S. aureous and Escherichia coli respectively by disk inhibition assay. While other both extracts also showed significant activity. Antioxidant activity was performed through 1, 1-diphenyl-2-picrylhydrazyl scavenging free radical assay which showed that all extracts showed significant (p<0.05) activity. Methanolic extract showed highest antioxidant activity (68.33%) followed by aqueous extract (53.18%), while ethanolic extract showed lowest antioxidant activity (39.47%). Total phenolic and flavonoid contents were determined by spectrophotometer assay which showed that aqueous extract contain highest phenolic and flavonoid contents as 230 mg/g equ G.A and 137 mg/q equ QE. The characterization of phenolic compounds was done by high performance liquid chromatography which revealed the presence of chromatotropic acid, quercitin, gallic acid, syringic acid, ferulic acid, trans 4-hydroxy-3-methoxy cinamic acid and vinillic acid are the major phenolic in all extracts.