International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Effect of hydrocarbons (Gasoil and spent motor oil) on biochemical parameters of barley (Hordeum vulgare)

By: Ahmed Mortet, Abdelwaheb Chibani, Houcine Abdelhakim Reguieg Yssaad

Key Words: Gasoil, Spent motor oil, Hordeum vulgare, Biochemical parameters

Int. J. Biosci. 11(5), 176-185, November 2017.


Certification: ijb 2017 0143 [Generate Certificate]


Petroleum compounds are one of the most commonly encountered pollutants in soils, presenting a stressful environmental factor for plants. The purpose of this work is to study the effect of hydrocarbons (gasoil and spent motor oil) on some biochemical parameters of barley Hordeum vulgare. Plantlets were sown in five different concentrations of gasoil (0%, 0.125%, 0.25%, 0.375% and 0.5%) and spent motor oil (0%, 1.25%, 2.5%, 3.75% and 5%) for 75 days in a greenhouse. The results show a significant reduction in the levels of chlorophyll with the gradual increase of gasoil and spent motor oil, noting that the lowest values were obtained at 0.5% gasoil (with a reduction of 59%) and 5% spent motor oil (with 73% of decrease compared to control). The same decrease was observed for proteins and soluble sugars recording that the content of leaves was higher than those of roots. The lowest contents (10.04 mg.g-1 of proteins and 14.53 mg.g-1 of soluble sugars) were registered at 0.5% and 0,375% gasoil respectively in roots. In addition, the same doses led to a significant increase in proline contents in comparison with the control, recording the highest levels (4.94 and 6.29 mg.g-1 dry matter in roots, and 11.97 and 16.78 mg.g-1 dry matter in leaves) for the highest doses of gasoil and spent motor oil respectively. What does it mean that gasoil and spent motor oil have an influence on stability of biochemical parameters of barley.

| Views 37 |

Effect of hydrocarbons (Gasoil and spent motor oil) on biochemical parameters of barley (Hordeum vulgare)

Achuba FI. 2006. The effect of sublethal concentrations of crude oil on the growth and metabolism of Cowpea (Vigna unguiculata) seedlings. Environmentalist 26, 17-20.

Alkio M, Tabuchi TM, Wang X, Colón-Carmona A. 2005. Stress responses to polycyclic aromatic hydrocarbons in Arabidopsis include growth inhibition and hypersensitive response-like symptoms. Journal of Experimental Botany 56(421), 2983-2994.

Baker JM. 1970. The effects of oils on plants. Environmental Pollution 1(1), 27-44.

Boggess SF, Aspinall D, Paleg LG. 1976. Stress metabolism. IX. The significance of end product inhibition of proline synthesis and of compartmentation in relation to stress-induced proline accumulation. Australian Journal of Plant Physiology 3(4), 513-525.

Bradford M. 1976. A rapid and sensitive method for the quantotation of protein utilizing the principe of protein-dye briding. Analytical Biochemistry 72, 248-254.

Cheikh H, Hamed R, Abddellaoui K, Kadri M, Ben Naceur S, Bel Hadj. 2008. Evaluation de la tolérance au stress salin de quelques accessions d’orge (Hordeum vulgare L.) Cultivées en Tunisie. Sciences et Technologie 28, 30-37.

Cunnigham SD, Berti WR. 1993. Remediation of contaminated soils with green plants: An overview. In vitro Cellular and Developmental Biology-Plant 29(4), 207-212.

Diab EA. 2008. Phytoremediation of oil contaminated desert soil using the rhizosphere effects. Global Journal of Environmental Research 2(2), 66-73.

Dubois M, Gilles KA, Hamilton JK, Rebers PA,

Smith F. 1956. Calorimetric method for determination of sugars and related substances. Analytical Chemistry 28(3), 350-356.

Edema NE, Obadoni BO, Erheni H, Osakwuni UE. 2009. Eco-Phytochemical  studies of plants in a crude oil polluted terrestrial habitat located at Lwhrekan, Ughelli North Local Government Area of Delta State. Nature and Science 7(9), 49-52.

Gao Y, Collins C. 2009. Uptake Pathways of Polycyclic Aromatic Hydrocarbons in White Clover. Environmental Science and Technology 43(16), 6190‑6195.

Hassani A, Dellal A, Belkhodja, Kaid-Harche M. 2008. Effet de la Salinité sur l’eau et certains osmolytes chez l’orge (Hordeum Vulgare L.). European Journal of scientific Research 23(1), 61-69.

Hoagland DR, Arnon DI. 1938. The water-culture method for growing plants without soil. california agricultural experiment station publications; ucdavisamericana  347, 1-39.

Janmohammadi M, Bihamta MR, Ghasemzadeh F. 2013. Influence of rhizobacteria inoculation and lead stress on the physiological and biochemical attributes of wheat genotypes. Cercetâri Agronomice în Moldova. XLVI, No. 1, 153.

Jones DL, Hodge A, Kuzyakov Y. 2004. Plant and mycorrhizal regulation of rhizodeposition. New Phytologist 163, 459-480.

Khan AG. 2005. Role of Soil Microbes in The Rhizospheres of Plants Growing on Trace Metal Contaminated Soils in Phytoremediation. Journal of Trace Elements in Medicine and Biology 18, 355-364.

Kuboi T, Noguchi A, Yazaki J. 1986. Family-depended cadmium accumulation characteristic in higher plants. Plant Soil 92, 405.

Leahy JG, Colwell RR. 1990. Microbial Degradation of Hydrocarbons in the Environment. Microbial Review 54(3), 305-315.

Levingneron A, Lopez F, Vansuyt G, Berthomieu P, Poureroy P, Casse-Delbart F. 1995. Les plantes face au stresse salin. Cahier agricultures 4, 263-273.

Luepromchai E, Lertthamrongsak W, Pinphanichakarn P, Thaniyavarn S, Pattaragulwanit K, Juntongjin K. 2007. Biodegradation of PAHs in petroleum contaminated soil using tamarind leaves as microbial inoculums. Songklanakarin Journal of Science and Technology 29(2), 515-527.

Malallah G, Afzal M, Gulshan S, Kurian M, Dhami MSI. 1996. Vicia faba as a bioindicator of oil pollution. Environmental Pollution 92, 213-217.

Malallah G, Afzal M, Kurian M, Gulshan S, Dhami MSI. 1998. Impact of oil pollution on some desert plants. Environment International 24, 919-924.

Minai-Tehrani D, Tavakoli Temah A, Rashidfarokhi A, Noormohammadi A, Khodakarami A, Talebi M. 2012. The effect of light crude oil contaminated soil on the growth and germination of Sorghum bicolor. In: Dobránszki J (Ed) Sorghum. The European Journal of Plant Science and Biotechnology 6, 81-84.

Minai-Tehrani D. 2008. Effect of heavy crude oil contaminated soil on germination and growth of Poa trivialis (rough meadow-grass). Archives of Agronomy and Soil Science 54, 83-92.

Monneveux PH, Nemmar M. 1986. Contribution à l’étude de la résistance à la  sécheresse chez le blé tendre (Triticum aestivum L.) et chez le blé dur (Triticum durum Desf.): Etude de l’accumulation de la proline au cours du cycle de développement. Agronomie, 6(6), 583-590.

Morris LC, Thompson JF, Johnson CM. 1969. Metabolism of glutamic and N-acetyl glutamic acid in leaf discs and cell-free extracts of higher plants. Plant Physiology 44, 1023-1026.

Nwaogu LA, Onyeze GOC, Nwabueze RN. 2008. Degradation of diesel oil in polluted soil using Bacillus subtilis. African Journal of Biotechnology 7(12), 1939-1943.

Odat S, Alshammari AM. 2011.  Seasonal  Variations  of  Soil  Heavy  Metal  Contaminants  along  Urban Roads : A  Case  Study  from  the City  of  Hail,  Saudi  Arabia. Jordan Journal of Civil Engineering. 5(4), 581-591.

Porra RJ. 2002. The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b. Photosynthesis Research 73, 149-156.

Rayapati PJ, Stewart CR. 1991. Solubilization of  proline dehydrogenase from maize (Zea mays L.) mitochondria. Plant Physiology 95, 787-791.

Stewart CR, Boggess F, Aspinall D, Paleg IG. 1977. Inhibition of proline oxidation by water stress. Plant Physiology 59, 930-932.

Weisman WH. 1998. Total Petroleum Hydrocarbon Criteria Working Group Series Vol.1: Analysis of petroleum hydrocarbons in environmental media. Amherst Scientific Publishers. Massachusetts.

Wyszkowski M, Ziolkowska A. 2008. Effect of Petrol and Diesel oil on content of organic carbon and mineral components in soil. American-Eurasian Journal of Sustainable Agriculture 2(1), 54-60.

Ahmed Mortet, Abdelwaheb Chibani, Houcine Abdelhakim Reguieg Yssaad.
Effect of hydrocarbons (Gasoil and spent motor oil) on biochemical parameters of barley (Hordeum vulgare).
Int. J. Biosci. 11(5), 176-185, November 2017.
Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
innspub logo
english language editing
    Publish Your Article
    Submit Your Article
Email Update