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Detection of natural infection and reaction of tomato lines to potato virus Y in Pakistan

By: Adnan Ahmad, Muhammad Ashfaq, Tariq Mukhtar, Saad Imran Malik, Irfan Anwer, Muhammad Ahsan, Tazeem Riaz

Key Words: Solanum lycopersicum, Potato virus Y, Molecular detection, Screening

Int. J. Biosci. 11(1), 343-350, July 2017.

DOI: http://dx.doi.org/10.12692/ijb/11.1.343-350

Certification: ijb 2017 0038

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Abstract

Tomato (Solanum lycopersicum L.) is an important solanaceous crop worldwide including Pakistan. In Pakistan the successful production of tomato is hampered due to many viral diseases including PVY which causes havoc and colossal yield losses. For management of plant viruses, accurate and proper identification of plant viruses and resistance sources is very significant. In present study, a total of 595 tomato samples with symptoms like mosaic, vein chlorosis and mild mottling were collected from tomato fields in Pakistan. All symptomatic samples were screened for the presence of Potato virus Y by DAS-ELISA using virus specific polyclonal antiserum (Bioreba AG, Switzerland). Among symptomatic samples, 104 were positive for PVY infection, of which, only eight were further screened for the presence of PVY by RT-PCR using primer pair PVYPK-F/R, that resulted in amplification of 1050 bp fragments. A total of 1050 nucleotides were obtained by sequencing each amplicon comprising a full length coat protein gene including 300 bases of UTR. The sequences of two isolates were submitted to Genbank under accession number KX816568 and KX816570. The isolate AARTPK (KX816568) was used in screening of 11 tomato cultivars. The cultivars; Kalam, NSC-92, Yaqui were found resistant (R), Rio-grandi as moderately resistant (MR) and Super-SPC and Giant-cluster as moderately susceptible (MS). Similarly, the response of BSS-30 and Gala was recorded as susceptible (S) and of Junny-2144, CKD-267 and Jagular as highly susceptible (HS). The identified resistant cultivars can be used as genetic source in developing resistant varieties against PVY in future.

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Detection of natural infection and reaction of tomato lines to potato virus Y in Pakistan

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Adnan Ahmad, Muhammad Ashfaq, Tariq Mukhtar, Saad Imran Malik, Irfan Anwer, Muhammad Ahsan, Tazeem Riaz.
Detection of natural infection and reaction of tomato lines to potato virus Y in Pakistan.
Int. J. Biosci. 11(1), 343-350, July 2017.
http://www.innspub.net/ijb/detection-natural-infection-reaction-tomato-lines-potato-virus-y-pakistan/
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