International network for natural sciences – research journal
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Decolorization of mono azo dye methyl orange with Epilithon biofilm: effects of physico-chemical parameters

By: Sadaf Shabbir, Muhammad Faheem, Philip G. Kerr, Naeem Ali, Yonghong Wu

Key Words: Decolorization, Methyl orange, Epilithon, Biofilm, Azo dye.

Int. J. Biosci. 9(3), 12-22, September 2016.

DOI: http://dx.doi.org/10.12692/ijb/9.3.12-22

Abstract

Azo dyes are one of the most widely used class of dyes in different industries. These dyes are generally recalcitrant under natural aquatic and conventional wastewater treatment systems. The study is innovative in demonstrating the role of aquatic biofilm, epilithon, in removal of an azo dye, methyl orange, under varying environmental conditions in a laboratory set up. Epilithon biofilm was collected by peeling it from the surface of submerged rocks in Xuan Wu Lake, Nanjing, China. The biofilm was immobilized on the surface of specific bio-carriers (AAM carriers). The attached biofilms showed 50->99.9% removal of dye at a concentration of 25-500 mg L-1 in 24-172 hrs. A maximum dye removal rate was observed at pH 7 using 0.8 mg L-1 biomass under mesophilic temperature (30 ˚C).

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