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Cytotoxic effects of aqueous and methanolic extracts of Incarvillea emodi (Royle Ex Lindl.) Chatterjee on mammalian cells

By: Yasir Ihtesham, Uzma Khan, Wajiha Khan, Ikram Ullah

Key Words: Incarvillea emodi, Cytotoxic effects, CHO-K1 cell line.

Int. J. Biosci. 9(2), 104-109, August 2016.

DOI: http://dx.doi.org/10.12692/ijb/9.2.104-109

Abstract

The present study is designed to investigate the comparative cytotoxic activity of crude aqueous and methanolic extracts of different parts (aerial parts with flowers, roots and aerial parts with fruits) of Incarvillea emodi collected from different Himalayan regions in Pakistan, against CHO-K1 (Chinese hamster ovary cell line). In vitro cytotoxic assay was performed by the crystal violet assay. Cells were suspended with trypsin/EDTA in 10 % calf serum. Hemocytometer was used for cell counting. The absorbance was measured using a microplate reader at a wavelength of 562 nm and percentage growth inhibition was calculated. The crude aqueous and methanolic extract (1-200 µg/ml concentration) of Incarvillea emodi was taken for cytotoxic activity. At highest concentration i.e. 200µg/ml percentage growth inhibition was recorded above 90% for all the six samples of the plant. The results showed that cell growth is significantly lower in extract treated cells compared to untreated control. Based on the results it is determined that Incarvillea emodi is a significant source of biologically active substances that have cytotoxic activity in vitro.

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