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Callus induction and indirect organogenesis in Chrysanthemum morifolium Ramat

By: Rawnak Jahan Swarna, DilrubaYeasmin, Md. Mostafizur Rahman, Md. Firoz Alam

Key Words: Chrysanthemum morifolium, leaf segments, indirect organogenesis.

Int. J. Biosci. 9(3), 139-149, September 2016.

DOI: http://dx.doi.org/10.12692/ijb/9.3.139-149

Abstract

A procedure for callus induction and indirect organogenesis ofChrysanthemum morifoliumRamat.using leaf segments as explant has been developed. For callus induction, leaf segments were cultured in MS medium (Murashige and Skoog’s, 1962) supplemented with 2,4-D singly (0.1, 0.5, 1.0, 1.5, 2.0 mg/litre) and IAA (0.5mg/litre) in combination with BAP (0.1, 0.2, 0.5, 1.0, 1.5 mg/litre). Effective callus induction and growth were obtained in the medium of MS supplemented with 2.0 mg/l 2,4-D and0.5 mg/l IAA + 0.2 mg/l BAP. 90% callus induced in both medium. Induced callus was transferred to the regeneration medium for effective shoot regeneration in 1.5 mg/l NAA + 1.0 mg/l BAP combination. The shoot regeneration frequency reached 90%. In order to induce roots, the shoots were transferred to the root induction media, and 1.0 mg/l IBA was recorded for maximum (100%) number of root induction on calli regenerated shoots.So, the indirect organogenesis of Chrysanthemum morifolium Ramat.was achieved through callus cultures and it may be possible to regenerate a plant from genetically variable cells of the callus tissue.

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