Application of chitosan and Trichoderma against soil-borne pathogens and their effect on yield of tomato (Solanum lycopersicum L.)
By: Nusrat Jahan Nitu, Md. Mahidul Islam Masum, Rayhanur Jannat, Salma Sultana, Md. Khurshed Alam Bhuiyan
Key Words: Trichoderma-fortified compost, Chitosan, Root diseases, Biological control, Tomato.
Int. J. Biosci. 9(1), 10-24, July 2016.
Plant diseases need to be controlled to uphold the quality and load of yields produced by grower’s across the world. A variety of approaches may be used to halt, alleviate or control plant disease. Uses of different biological control agents regulate the growth of plants in a miraculous form. The present study was conducted to reduce soil-borne diseases caused by Fusarium oxysporum f. sp. lycopersici, Sclerotium rolfsii, Rhizoctonia solani and to increase the yield of tomato field under natural epiphytotic conditions through the application of Trichoderma harzianum and chitosan based-treatments at different methods. Isolate T. harzianum pb27 displayed 88.56%, 90.58%, 89.28% inhibition rate and chitosan 800 ppm showed 78.61%, 81.11%, 77.22% inhibition rate on PDA plate against the highly virulent isolate of F. oxysporum f.sp. lycopersici (FOL), S. rolfsii, and R. solani, respectively. Trichoderma-fortified compost along with foliar spray of chitosan and seedling roots dipped both in chitosan solution and Trichoderma spore suspensions appeared to be best in controlling the post-emergence seedling mortality (81.83%) caused by soil-borne pathogens. Similarly the same treatment was also found the most promising for the management of different soil-borne diseases of tomato including Fusarium wilt caused by F. oxysporum f. sp. lycopersici, collar rot/southern blight caused by S. rolfsii, dry root rot or wet root rot caused by R. solani. In addition, all treatments significantly increased yield and yield contributing components of tomato. Therefore, integration of two or three components is advised as one of the most worthy methods in order to effectively control the disease and improve crop performance.