International network for natural sciences – research journal
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Antibiotic resistance pattern and gender wise prevalence of Pseudomonas aeruginosa strain isolated from the tertiary health care units

By: Shahida Mangi, Anwar Hussain Phulpoto, Muneer Ahmed Qazi, Nisar Ahmed Kanhar

Key Words: P. aeruginosa, Multidrug resistance, Antimicrobial sensitivity profiling, Clinical samples, Public and private health care units.

Int. J. Biosci. 9(5), 173-182, November 2016.

DOI: http://dx.doi.org/10.12692/ijb/9.5.173-182

Abstract

Antibiotic resistance is a matter of great concern and it has finally attracted the attention of scientists and mass-media worldwide. The present study aims to determine the gender wise prevalence and multidrug resistance in P. aeruginosa isolated from clinical samples of public and private health care units. The 280 clinical samples (118 female and 162 male) of pus, urine, pleural fluid, blood, high vaginal swabs, throat, ear, stool, cerebrospinal fluid and ascitic fluid were collected from public and private health care hospitals of Khairpur and Sukkur cities of Pakistan. The isolates were isolated and identified using conventional microbiological methods and molecular characterization using 16S rRNA gene sequence homology. The antimicrobial sensitivity was determined using Kirby-Bauer’s disc-diffusion method according to the clinical laboratory standard institute. The Overall 28% samples were found positive for the isolation of P. aeruginosa and highest 100% prevalence was found in ascitic fluid, followed by ear swab 50% while the lowest percent prevalence was in urine (3.8%) samples. The overall prevalence was found more in male patient than female patients. The results of antibiotic sensitivity profiling revealed complete resistance against all the antibiotics tested. The phylogenetic correlation of amplified 16S rRNA gene sequence of isolate shared 99% similarity with P. aeruginosa strain (Gen Bank accession no. JF513146.1). Increasing multidrug resistance among Pseudomonas is an alarming situation in a hospital setting and requires prompt steps to cope up with resistance.

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