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Antibacterial and antifungal activity of Cannabis sativa L., Mentha longifolia L. and Ricinus communis L.

By: Imran Khan, Muhammad Sabir

Key Words: Antibacterial, Zone of inhibition, Antifungal, Cannabis sativa, Mentha longifolia, Ricinus communis.

Int. J. Biosci. 9(4), 169-175, October 2016.

DOI: http://dx.doi.org/10.12692/ijb/9.4.169-175

Abstract

Bacterial and Fungal diseases still possesses a serious threat to public health. In this study the antibacterial and antifungal potential of crude methanolic extracts of Cannabis sativa (C. sativa), Mentha longifolia (M. longifolia) and Ricinus communis (R. communis) was investigated. The antibacterial activity was determined using agar well diffusion method against two gram positive i.e. Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis) and gram negative bacterial strains i.e. Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa). The antifungal activity was invistigated by using agar tube dilution method aginst two fungal strains i.e. Aspergillus niger (A. niger) and Aspergillus flavous (A. flavous). In antibacterial activity, all the plants extracts showed higher bacterial inhibition against gram negative strains compared to gram positive strains. E. coli was recorded being the most susceptible strain while, B. subtilis and P. aeruginosa was the most resistant strains. R. communis showed significant antibacterial potential with maximum zone of inhibition (ZOI) of 28mm against E. coli and minimum ZOI of 8mm against B. subtilis and P. aeruginsa. For C. sativa, maximum and minimum ZOI of 26mm and 8mm was recorded against E.coli, B. subtilis and P. aeruginsa. M. longifolia showed maximum of 24mm ZOI against E. coli while, 8mm minimum ZOI was recorded against B. subtilis and P. aeruginsa strains. In antifungal activity, R. communis was the most active plants extract with 26.72% and 25.40% fungal inhibition against A. niger and A. flavous. Furthermore, C. sativa showed 18.10% and 18.96% fungal inhibition against A. niger and A. flavous, while for M. longifolia 16.37% and 19.82% fungal inhibition was recorded against A. niger and A. flavous respectively. These results showed that the methanolic leaves extract of R. communis possessed a good antibacterial and antifungal potential as compared to C. sativa and M. longifolia and can be consider in the bacterial and fungal control strategy.

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