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Anti-proliferative activity of Thuja occidentalis seed extract through regulation of BAX and BCL-X gene exprssion

By: Md. Shihabul Islam, Md. Sifat Rahi, Israt Jerin, Kazi Ebnul Hasan, Salek Ahmed Sajib, KMKB Ferdaus, Kazi Md. Faisal Hoque, Md. Abu Reza

Key Words: Anticancer, Phytochemical, EAC cells, Hemmagglutination assay, Apoptosis, BAX, BCL-X.

Int. J. Biosci. 11(4), 372-386, October 2017.

DOI: http://dx.doi.org/10.12692/ijb/11.4.372-386

Certification: ijb 2017 0169 [Generate Certificate]

Abstract

Cancer is one of the most devastating diseases which involve uncontrolled cell growth. The conventional treatments of cancer have strong side effects thus scientists are seeking safer treatment option from nature. Various natural plants are well documented for having anti-proliferative activity with minimal side effects. Thuja occidentalis is one of the common medicinal plants in our country which is successfully used as homeopathic medicine since many years to cure several diseases including wart. However, so far there is no proper scientific investigation to demonstrate these medicinal properties of this important plant. Therefore, the present investigation was designed to evaluate the anticancer, antioxidant, cytotoxic and antibacterial activity of the crude extract of the T. occidentalis seed. Phytochemical examinations were carried out to assess the types of secondary metabolites present in extract. Haemaglutination assay was performed to determine the presence of lectin protein which is considered as an effective anticancer agent. DPPH free radical scavenging assay was carried out to determine the antioxidant activity of the extract where it was compared with the ascorbic acid. EAC (Ehrlich ascites carcinoma) cells bearing mice were treated with 50mg/Kg/day of crude extract. Cancer cell growth inhibition was calculated by hemocytometric cell counting which compared with control after the five days of treatment. Morphological changes indicative of apoptosis were evident in EAC cells which were observed using optical microscopy after DAPI staining. RNA was extracted from both control and treated cells from mice peritoneum and cDNA was prepared to observe the expression of the cancer related gene such as BAX and BCL-X by PCR amplification which specifies apoptosis. Disc diffusion method was used to measure the bacterial susceptibility to crude extract. Our research suggests that T. occidentalis seed extract have anti-proliferative activity and strong antioxidant activity which can be a reliable and safer treatment option of cancer.

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Anti-proliferative activity of Thuja occidentalis seed extract through regulation of BAX and BCL-X gene exprssion

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Md. Shihabul Islam, Md. Sifat Rahi, Israt Jerin, Kazi Ebnul Hasan, Salek Ahmed Sajib, KMKB Ferdaus, Kazi Md. Faisal Hoque, Md. Abu Reza.
Anti-proliferative activity of Thuja occidentalis seed extract through regulation of BAX and BCL-X gene exprssion.
Int. J. Biosci. 11(4), 372-386, October 2017.
http://www.innspub.net/ijb/anti-proliferative-antioxidant-antibacterial-activity-seed-extract-thuja-occidentalis/
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