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Amperometric Glucose Biosensor Based on Activated Carbon/5-Methyl 1, HydroxyPhenazine/Glucose Oxidase Matrix

By: Sameen Yousaf, Maira Anam, Naeem Ali

Key Words: Biosensor, Glucose oxidase, 5-methyl 1-Hydroxy phenazine, Activated carbon, Immobilization temperature.

Int. J. Biosci. 9(6), 193-203, December 2016.

DOI: http://dx.doi.org/10.12692/ijb/9.6.193-203

Abstract

Recently, advance bio-sensing devices have given considerable importance, because of their capability to identify the target compounds promptly. In this context,2nd generation glucose biosensor has been fabricated using natural redox compound, 5-methyl 1, hydroxyphenazine (5-MHP)obtained from P. aeruginosa. Amperometric detection was based on interaction of glucose with the working electrode loaded with activated carbon (AC), 5-MHP and glucose oxidase (GOx), AC/5-MHP/GOx. Working electrodes prepared at three different temperatures 4oC, 25oC and 47oC, sensitively detect glucose andshowed the linear ranges of R2 = 0.98, R2 = 0.98 and R2 = 0.99 respectively with detection limit of 0.3┬ÁM at signal-to-noise ratio (S/N)=3. It was found that GOx immobilization temperature directly influence the long term efficiency of glucose biosensor. Electrode fabricated at 4oC exhibited greater operational stability i.e. 74% followed by 25oC (68%) and 47oC (48%). Furthermore, theresponse timewith eachglucoseconcentration (2.0 to 26.0mM) was relatively less i.e. 2s, for enzyme electrode fabricated at 4oC whereas it was 4s and 5s for working electrodes prepared at 25oC and 47oC respectively.

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