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Adaptation and optimization of various physico-chemical conditions for pyrene degradation by bacteria isolated from the Rhizospheric soil of Morus alba

By: Nadia Bibi, Mohammad Hamayun, Anwar Hussain, Aaqib Sayyed, Sumera Afzal Khan

Key Words: Adaptation, Physico-chemical parameter, Optimization, Biodegradation

Int. J. Biosci. 10(4), 343-353, April 2017.

DOI: http://dx.doi.org/10.12692/ijb/10.4.343-353

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Abstract

Isolation from contaminated sites using classical enrichment technique is of common practice and give promising results but with a narrow range of microbial community. In this study, wild bacteria were isolated and adopted through changing growth media and exposure to UV-light for 15minutes. Various physico-chemical conditions were optimized for pyrene degradation by isolate M3 with 1000ppm concentration, optimum temperature of 35°C, pH 7 and shaking speed of 120 rpm. Isolate M3 degraded 76.31% pyrene with 0.970 OD value and 2.7×10-31 CFUmL-1 after 120 hours. Metabolites formed during degradation process on comparison with authentic standards were identified as phenanthrene, catechol, salicylaldehyde and salicylic acid. Plasmid isolation and curing showed a fractional reduction in pyrene degradation by this isolate. Experiment was performed in triplicate and data was analysed by 2-way ANOVA and level of significance 0.002.

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Adaptation and optimization of various physico-chemical conditions for pyrene degradation by bacteria isolated from the Rhizospheric soil of Morus alba

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Nadia Bibi, Mohammad Hamayun, Anwar Hussain, Aaqib Sayyed, Sumera Afzal Khan.
Adaptation and optimization of various physico-chemical conditions for pyrene degradation by bacteria isolated from the Rhizospheric soil of Morus alba.
Int. J. Biosci. 10(4), 343-353, April 2017.
http://www.innspub.net/ijb/adaptation-optimization-various-physico-chemical-conditions-pyrene-degradation-bacteria-isolated-rhizospheric-soil-morus-alba/
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