Pseudomonas fluorescens as plant growth promoting Rhizo-Bacteria and biological control agents for white rust disease in chrysanthemum
By: Hanudin, Kurniawan Budiarto, Budi Marwoto
Key Words: P. flourescens, PGPR, white rust Puccinia horiana, biological control, in vitro and in vivo testing.
Int. J. Agr. Agri. Res. 8(4), 22-34, April 2016.
The use of plant growth promoting rhizobacteria (PGPR) to control disastrous diseases in many crops has been considered important recently. The research was conducted to evaluate several bacterial strains to control white rust in chrysanthemum. The research consisted of two chronological experiments, in vitro and in vivo testing of bacterial isolate against the disease. 16 bacteria isolates were collected, purified and applied on the rust-infected leaf. Three isolates showed more effective in suppressing white rust during in vitro testing and further identification confirmed these strains, Pf Kr 2, Pf Smd 2 and Pf Ktl were grouped into P. flourescens. In vivo testing of the Pf isolates also revealed consistent performances of these three Pf isolates in retarding the growth of fungal Puccinia horiana and even more effective than Azotobacter sp. and Azospirilium sp. The production of ethylene on the leaf was coincidence with the slower development and lower disease intensity on the treated plants. Among the three strains, Pf Kr 2 showed stronger suppression to the disease. Further investigations are needed to further elucidate the existence of specific interrelation between Pf strains and plant genotypes or cultivars. Prior to a selection of good bacterial inoculants, it is recommended to select cultivars that benefit from association with these bacteria.