Investigation of GA3 effect on in vitro micro propagation of potato varieties
By: Azhar Mehmood, Abbas Hussain Shah, Azhar Hussain Shah, Muhammad Sajid, Habib Ahmad
Key Words: GA3, Potato, Nodal culture, MS media.
Int. J. Agr. Agri. Res. 9(2), 190-197, August 2016.
In this investigation MS media supplemented with different concentrations of GA3 viz. 0.00, 0.12, 0.25, 0.50, 0.75mg/L were used for in vitro micro propagation of potato varieties Cardinal and Desiree. The parameters included were percent sprout, plantlet height, number of nodes per plantlet, number of days to root initiation, and number of days to transferable height of the plantlets. Statistical analysis showed that the varieties, GA3 concentration and varieties vs GA3 concentration were highly significant for plant height, number of nodes per plantlet and number of days to transferable height of the plantlets. Plantlets developed in M.S media supplemented with 0.25 mg/L of GA3 for Desiree and 0.12mg/L of GA3 for Cardinal produce maximum plant height, more number of nodes, reduced no. of days to root initiation and took less number of days to transferable height of the plant. Plantlets developed in these treatments were healthy and vigorous than the plantlets developed in other treatments. The results have shown that sprout percentage was high in variety Cardinal and Desiree proved efficient in response of no. of days to transferable height of the plant.