International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Evaluation of the insecticidal properties of fractionated extracts of Ocimum canum and Laggera pterodonta on stored maize against the infestation of Sitophilus zeamais (Coleoptera: Curculionidae)

By: D. J. Langsi, E. N. Nukenine, C. N. Fokunang, J. W. Goudoungou, D. Kosini, T. G. Fotso, Tchinda, G. Agbor

Key Words: Botanical, Fractionated extract, Progeny production inhibition, Repellence, Toxicity

Int. J. Agron. Agri. Res. 10(6), 41-50, June 2017.

Abstract

The weevil, Sitophilus zeamais (Motschulsky), is a devastating pest of stored maize, especially in the warm humid tropical regions. The objective of this study was to test the insecticidal properties of fractionated Ocimum canum and Laggera pterodonta extracts used as natural alternatives to synthetic insecticides in the management of maize S. zeamais weevils. Fractionated extracts were obtained by maceration of the leaves of O. canum and Laggera pterodonta. Cyclohexane, acetone and methanol were used as extraction solvents and the tests were carried out at 1, 5 and 10 g/kg on adult S. zeamais, for mortality, progeny production inhibition, feeding deterrence and repellence with a control that received no extract treatment. Adult mortality was recorded within 1, 7 and 14 days post-exposure. All the fractions showed insecticidal activity that increased with dosage and period of post-treatment. At 10 g/kg the Laggera methanol fraction caused a mortality of 20 % within 14 days of exposure, and a progeny production inhibition rate of over 84 %. Laggera Cyclohexane fraction (5 g/kg) that showed repellence index of 75.44 % greater than neem oil (Azadirachta indica) that served as the positive control referenced plant.

| Views 40 |

Evaluation of the insecticidal properties of fractionated extracts of Ocimum canum and Laggera pterodonta on stored maize against the infestation of Sitophilus zeamais (Coleoptera: Curculionidae)

Abbott WS. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18, 266-267.

Adjou ES,  Kouton S,  Dahouenon-Ahoussi E, Sohounhloue DCK, Soumanou MM. 2012. Antifungal activity of Ocimum canum Essential oil against Toxinogenic Fungi isolated from Peanut Seeds in post-harvest in Benin, International Research Journal of Biological Sciences 1(7), 20-26.

AFNOR. 1982. Recueil de normes franc ¸aise des produits dérivés des fruits et légumes. Association franc ¸aise de normalisation, 1st edition. AFNOR, Paris. 27 p.

Akob CA, Ewete FK. 2010. Effect of Four Mid-Altitude Maize Varieties on Oviposition, Development and Sex Ratio of Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae) African Entomology 18(2), 253-258. 2010.

Asawalam EF, Emosairue SO, Hassanali A 2008. Constituents of the Essential Oil of Vernonia amygdalina as Maize Weevil Protectants.  Tropical and Subtropical Agroecosystems 6(2), 95-102.

Bosque-Perez NA. 1992. Major insect pests of maize in Africa:  biology and control. IITA Research Guide 30, IITA Training Programme, International Institute of Tropical Agriculture (IITA), 28.

Burkill HM. 1985. The Useful Plants of West Tropical Africa. Vol. 1, Families A-D. Royal Botanic Gardens Kew, 479-481.

Christos GA, Nickolas GK, Pasquale T. 2006. Responses of Sitophilus oryzae (Coleoptera: Curculionidae) and Tribolium confusum (Coleoptera: Tenebrionidae) to traps baited with pheromones and food volatiles. European Journal of Entomology. 103, 371–378.

CIMMYT, IITA 2010. Maize – Global alliance for improving food security and the livelihoods of the resource-poor in the developing world. Draft proposal submitted by CIMMYT & IITA to the CGIAR Consortium Board. El Batan, Mexico. 91 p.

Dobie P, Haines CP, Hodges RJ, Prevett PF. 1984. Insects and Arachnids of Tropical Stored Products their Biology and Identification. London. U.K. 273 p. Domain site:

www.indjst.org.

Egharevba HO, Kunle FO, Okwute SK, Okogun JI. 2012.  Chemical Constituents of the Essential Oil of Laggera pterodonta (DC.) Sch. Bip.  From North-Central Nigeria, Journal of Applied Pharmaceutical Science 02(08), 198-202.

FAO. 2011. Food and Agricultural organization of the United Nations: Rome. FAOSTAT for 2011.

FARA (Forum for Agricultural Research in Africa). 2009. Patterns of Change in Maize Production in Africa: Implications for Maize Policy Development, 8pages.

Hill DS. 2008. Pest of Crops in Warmer Climates and their Control. 704.  Spinger Science and Business Media, Britain.

Isman MB. 2006.  Botanica1    insecticides, deterrents, and repellents in modem agriculture and an increasingly regu1ated world. Annual Review of Entomology, 51, 45-66.

Kaushik N, Vir S. 2000. Variations in fatty acid and composition of neem seeds collected from the   Rajasthan State of India. Biochemical Social Transactions, 28(6), 880-882.

Luciana BS, Jorlan C da S, Bruno EP; Francisco FP, Kellen M, Lígia HA, Ana CSC,  Marize TLPP. 2013. Insecticide irritability of plant extracts against Sitophilus zeamais; African Journal of Agricultural Research, 8(11), 978-983.

Lyon F. 2000. Science Direct- World Development II. Trust, Networks and Norms: The creation of social capital in agricultural economies in Ghana.  Journal of Stored Product Research, 28(4), 663-681.

Maribet Parugrug L, Aurea Roxas C. 2008. Insecticidal action of five plants against maize weevil, Sitophilus zeamais Motsch. (Coleoptera: Curculionidae) KMITL Science and Technology Journal 8(1).

Martins AP, Salgueiro LR, Vila R. 1999. Composition of the essential oils of Ocimum canum, O. gratissimum and O. minimum. Plant Medicines, 65(2), 187-189. 24.

Mazen A. 2012. Impact of Flavonoids against Woolly Apple Aphid, Eriosoma lanigerum (Hausmann) and Its Sole Parasitoid, Aphelinus mali (Hald.) Journal of Agricultural Science 4( 2), 2012.

McDonald LL, Guyr H, Speire RR. 1970. Preliminary evaluation of new candiolate materials as toxicants, repellent and attracts against stored product insect marketing Research. 189 p.

Mikolo B, Massamba D, Matos L, Lenga A, Mbani G, Balounga P. 2007.  Conditions de stockage et revue de l’entomofaune des denrées stockées du Congo-Brazzaville. Journal des Sciences, 7(1), 30-38.

Naghibi F, Mosaddegh M, Motamed SM, Ghorbani A. 2005.  Labiatae family in folk medicine in Iran: from ethnobotany to pharmacology. Iran Journal of Pharmaceutical Research, 2, 63-79.

Napoleao TH, Belmonte BDR, Pontual EV, Albuquerque de LP, Sa RA, Paiva LM, Coelho LCBB, Paiva PMG. 2013.  Deleterious effects of Myracrodruon urundeuva leaf extract and lectin on maize weevil, Sitophilus zeamais (Coleopera: Curculionidae). Journal of Stored Product Research, 54, 26-33.

Ngamo LST, Hance TH. 2007. Diversité des ravageurs des denrées et méthodes alternatives de lutte en milieu tropical. Tropicultura 25(4), 215-220.

Ngamo LST, Ngassoum MB, Jirovetz L, Adjoudji O, Nukenine EN, Moukala OE. 2001. Protection of stored maize against Sitophilus  zeamais  (Most) by  the use of essential oils of species from Cameroun. Med. Fac. Landbouww. Université Gentille, 66, 473-478.

Ngamo TLS, Goudoum A, Ngassoum MB, Noudjou WFC, Haubruge E, Lognay G, Kouninki H, Hance T. 2007a. Use of essential oils of aromatic plants as protectant of grains during storage. Agricultural Journal, 2(2), 204-209.

Ngamo TLS, Goudoum A, Ngassoum MB, Noudjou WFC, Haubruge E, Lognay G, Kouninki H, Hance T. 2007b.  Investigations on both chemical composition and insecticidal activities of essential oils of Vepris heterophylle (Rutaceae) from two localities of Northern Cameroon towards Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), Research Journal of Biological Sciences 2(1), 57-61.

Ngassoum MB, Ousmaila H, Ngamo LT, Maponmetsem PM, Jirovetz L, Buchbauer G. 2004. Aroma compounds of essential oils of two varieties of the spice plant Ocimum canum Sims from Northern Cameroon. Journal of Food Composition Analyses, 17(2), 197-204.

Njan Nlôga AM, Saotoing P, Tchouankeu JC,  Messi J. 2007. Effect of Essential Oils of Six Local Plants Used Insecticide on Adults of Anopheles gambiae, Giles 1902. Journal of Entomology 4(6), 444-450.

Nukenine EN. 2010. Stored products protection in Africa: Past, present and future. 10th International Working Conference on Stored Product Pests. Julius-Kühn-Archive 425, 26-41.

Nukenine EN, Adler C, Reichmuth C. 2007. Efficacy evaluation of plant powders from Cameroon as post-harvest grain protectants against the infestation of Sitophilus  zeamais  Motschulsky (Coleoptera: Curculionidae). Journal of Plant Diseases and Protection, 114(1), 30-36.

Nukenine EN, Monglo B, Awasom I, Tchuenguem FFN, Ngassoum MB. 2002. Farmers’ perception on some aspects of maize production, and infestation levels of stored maize by Sitophilus zeamais in the Ngaoundere region of Cameroon. Cameroon Journal of  Biological Sciences., 12, 18–30.

Nyarko AK, Asare-Anane H, Ofosuhene M, Addy ME, Teye K, Addo P. 2002. Aqueous extract of Ocimum canum decreases levels of fasting blood glucose and free radicals and increases antiatherogenic lipid levels in mice. Vasculuar Pharmacology 39(6), 273-279.

Osei-Akrasi K. 1999. Fumigation of Maize against Fungi and Insects with Smoke from Ocimum gratissimum (L) Leaf Powder.  B.Sc. Dissertation submitted to the Department of Crop and Soil Sciences, Faculty of Agriculture. Kwame Nkrumah University of Science and Technology, University Press Kumasi, Ghana.

Owusu EO, Akutse KS, Afreh-Nuamah K. 2008. Effect of some traditional plant components on the control of termites, macrotermes spp. (Isoptera: Termitidae) African Journal of Science and Technology (AJST) Science and Engineering Series 9(2), 82 – 89.

Prates HT,  Santos  JP, Waquil  JM, Fabris  JD, Oliveira AB, Forster  JE. 1998.  Insecticidal activity  of monoterpenes  against  Rhyzoperhta  domonica  (F.)  and Tribolium  castaneum  (Herbst).  Journal of Stored Product Research, 34(4), 243-249.

Quintin G, Katherine IN, Rachel IW. 2011 Smelling on  the  fly:  sensory  cues  and  strategies  for  olfactory navigation  in  Drosophila; Sciverse Science Direct Current  Opinion  in  Neurobiology  2012,  22, 216–222.

Rahman SS, Rahman M, Mohammad M, Rahman K, Shameem AB, Balaram R &. Fakruddin SMS. 2006. Ethanolic extract of melgota (Macaranga postulata) for repellency, insecticidal activity against rice weevil (Sitophilus oryzae). African Journal of Biotechnology 6(4), 379-383 P.

Regnault-Roger C, Hamraoui. 1993. Efficiency of plants from south of France used as traditional protectant of Phaseolus vulgaris L, against its  bruchid  Acantolides  obtectus Say: Journal of Stored  Products Research, 29, 259-264.

Statistical analysis systems 2003. SAS version 9.2 SAS Institute, Inc., Cary, North Carolina 321 P.

Talukder F, Howse P. 1994. Evaluation of Aphanamixis polystachya as a source of repellents, antifeedants, toxicants and protectants in storage against Tribolium castaneum (Herbst). Journal of Stored Products Research. 31(1), 55-61.

Throne JE, Eubanks MW. 2002. Resistance of Tripsacorn to Sitophilus zeamais and Oryzaephilus surinamensis. Journal of Stored Products Research 38(3), 239-245.

Vaddi HK, Ho PC, Chan YW, Chan CY. 2002. Terpenes in ethanol: haloperidol permeation and partition through human skin and stratum corneum changes. Journal of Controlled release 81, 121-133.

Vetrivel RA, Shanmugavalli N, Greety SC, Umashankar V. 2009. Hepatoprotective effects of Cassia tora on CCl4 induced liver damage in albino rats. Indian Journal of Science Technology 2(3), 41-44.

Vieira RF, Grayer RJ, Paton AJ. 2003. Chemical profiling of Ocimum americanum using external

flavonoids. Phytochemistry, 63(5), 555-567.

Wu Y, Wang F, Zheng Q, Lu L, Yao H, Zhou C, Wu X, Zhao Y. 2006. Hepatoprotective effect of total flavonoids from Laggera alata  against carbon tetrachloride-induced injury in primary cultured neonatal rat hepatocytes and in rats with hepatic damage. Journal of Biomedical Sciences 13, 569-578.

Xie YS, Bodnaryk RP, Fields PG. 1996.  A rapid and simple flour disc bioassay for test on substance active against stored product insects. The Canadian Entomologist 128, 865-875.

Zar JH. 1999. Bio statistical Analysis, 4th edition Prentice-Hall, Inc. Upper Saddle River, NJ, 662 p.

D. J. Langsi, E. N. Nukenine, C. N. Fokunang, J. W. Goudoungou, D. Kosini, T. G. Fotso, Tchinda, G. Agbor.
Evaluation of the insecticidal properties of fractionated extracts of Ocimum canum and Laggera pterodonta on stored maize against the infestation of Sitophilus zeamais (Coleoptera: Curculionidae).
Int. J. Agron. Agri. Res. 10(6), 41-50, June 2017.
http://www.innspub.net/ijaar/evaluation-insecticidal-properties-fractionated-extracts-ocimum-canum-laggera-pterodonta-stored-maize-infestation-sitophilus-zeamais-coleoptera-curculionidae/
Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update